Fig. 2.

Detection of Cell death by NST-732 in B16 melanoma cells in vitro; fluorescent microscopy, co-staining with propidium iodide (PI). A. Control cells; most cells are unstained, manifesting only blue auto-fluorescence (blue arrow), un-related to NST-732; few cells manifest binding of both NST-732 and PI (red arrow), reflecting naturally-occurring cell death in culture. B. Following incubation with BiCNU (100 µM, 16 h), numerous cells manifest uptake of NST-732 (green fluorescence). Some of the cells show uptake of NST-732, while excluding PI. These are cells in early apoptosis (EAC; green arrow). Some of the cells show uptake of both NST-732 and PI (red arrow), being cells in the late stages of apoptosis or undergoing a necrotic mode of cell death. Scale bar: 1 cm=80 µm