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. 2009 Dec 11;5(12):e1000769. doi: 10.1371/journal.pgen.1000769

Figure 3. HP1α and HP1γ inhibits chromatin remodeling in vitro.

Figure 3

(A) REA assay performed on a 5S polynucleosome template. Template at 1 nM was pre-incubated with indicated concentrations of recombinant F-HP1α (produced in baculovirus) before digestion by HhaI in the absence or presence of hSWI/SNF. At the end of the reaction (1hr), digestion products were separated on a 1% agarose gel. (B) REA assay performed as in (A) on a 202 bp mononucleosome template and the restriction enzyme PstI. Digestion products were separated on a 5% polyacrylamide gel. (C) REA assay performed as in (A) with indicated concentrations of recombinant F-HP1γ (produced in baculovirus). (D) Schematic representation of the HA-tagged HP1α point and deletion mutants produced in E. coli. Note that HA-HP1α(V22M) also carries a V21A mutation. (E) REA assays performed as in (A) with increasing concentrations of indicated HA-HP1α proteins. Values are averaged from 3 independent experiments.