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. 2009 Dec 11;5(12):e1000769. doi: 10.1371/journal.pgen.1000769

Figure 4. Brm and HP1α/HP1β have opposite effects on common target genes.

Figure 4

(A) Western blots of extracts from HeLa cells transfected with the indicated siRNA and used for the preparation of the RNAs used in (B,C). The blots shown are representative of experimental triplicates. (B) HeLa cells were transfected with Brm siRNAs. The mRNA abundance from the indicated genes was measured by RT–qPCR and normalized to levels of HPRT. Values were averaged from experimental triplicates and normalized to levels of HPRT mRNA. (C) As in (B), with the indicated HP1 siRNAs. (D) As in (B), using HeLa HA-HP1γ cells (see Figure 5) and HP1γ or Brm siRNAs and a treatment with 0.5 nM interferon-α2 for either 0 or 10 hours as indicated.