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. 2009 Dec 8;4(12):e8211. doi: 10.1371/journal.pone.0008211

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Teves et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Immunocytochemistry for protein Tyr-phosphorylation in the absence or presence of a 10 pM P gradient. ^A flagellum; ^B principal and end piece; ^C neck, principal and end piece; ^D punctuate staining of the acrosome region and flagellum; ^E equatorial band; ^F no label; ^G punctuate staining of the whole spermatozoon; ^H equatorial band and flagellum; ^I midpiece. Percentage of each identified pattern of Tyr-phosphorylation after treating the cells with (dark) or without (white) 10 pM P gradient in: (B) spermatozoa incubated under capacitating or (C), non-capacitating conditions. Data are expressed as the mean ± SEM. ^a Significant differences vs. without P (p<0.05).