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Effect of simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) treatment on chestnut allergens as analyzed by 12% SDS-PAGE (A) and IgE-immunoblotting (B). 1, Standard; 2, SGF-30 sec; 3, SGF-2 min; 4, SGF-10 min; 5, SGF-30 min; 6, SIF-15 min; 7, SIF-2 hr; 8, SIF-16 hr.
