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. 2009 Sep 23;20(1):78–86. doi: 10.1093/glycob/cwp149

Fig. 2.

Fig. 2

NSCs prepared from mouse striata (E14) and SVZs (P2, P10, P30, and adult) in the form of neurospheres. (A) Primary, secondary, tertiary, quaternary, and quinary neurospheres prepared from mouse striata (E14) or SVZs (P2, P10, P30, and adult). Scale bar represents 100 μm. (B) The proliferation rates of neurosphere-forming cells estimated by WST-8 assay at 0, 1, 3, and 5 days in vitro (n = 3). (C) The expression of NSC-specific genes in neurosphere-forming cells prepared from mouse striata (E14) or SVZs (P2, P10, P30, and adult) was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). “Brain” indicates the control cDNA prepared from the total RNA of adult mouse brains. “–RT” indicates negative controls without reverse transcription. β-Actin was detected as the control. (D) Expressions of nestin (NSC marker), β-III tubulin (neuron marker), GFAP (astrocyte marker), and GalC (oligodendrocyte marker) in neurosphere-forming cells and the differentiated cells cultured in the presence of 1% fetal bovine serum and the absence of FGF-2 and EGF. Nuclei were stained with H33258. Scale bar represents 100 μm. (E) The percentages of nestin+ cells in neurosphere-forming cells (n = 10).