Table 2.

MIC results and Erg11p substitutions for 25 C. albicans isolates with reduced susceptibility to fluconazole and 23 fluconazole-susceptible isolates by RCA^a and ERG11 sequencing.

		MIC (μg/ml)		Erg 11p amino acid substitutions
				D	D	E	F	F	G	G	G	G	K	K	R	S	V	V	Y
				1	2	2	1	4	3	4	4	4	1	1	4	4	4	4	1
Patient/isolate no.	Site	FLUa	VORa	1	7	6	4	4	0	4	6	6	2	4	6	0	3	8	3
				6	8	6	5	9	7	8	4	5	8	3	7	5	7	8	2
				E	E	D	L	S	S	E	S	S	T	R	K	F	I	I	H
Isolates with reduced fluconazole susceptibility
1b	Oropharynx	16	0.25			+	+											+
2b	Vagina	>256	0.03
3-Ab, c	Oropharynx	16	0.25			+	+											+
- Bb, c	Oropharynx	16	0.5			+	+											+
4d	Oropharynx	256	0.25			+								+				+
5d	Oropharynx	256	0.125						+
6-Ac, d	Oropharynx	256	>16			+
-Bc, d	Oropharynx	256	>16	+
7d	Oropharynx	256	>16			+					+			+				+
8-Ac, d	Oropharynx	256	0.5			+		+										+
-Bc, d	Oropharynx	256	1			+		+										+
9d	Oropharynx	256	>16			+											+
10d	Oropharynx	256	2			+										+		+	+
11d	Oropharynx	256	0.008			+											+
12-Ac, d	Oropharynx	256	2								+
-Bc, d	Sputum	32	0.5	+							+	+
13d	Vagina	32	0.016										+
14d	Blood	256	>16	+									+
15d	Bile	256	16														+
16-Ac, d	Oropharynx	16	0.125	+						+			+
-Bc	Oropharynx	256	2	+					+	+			+
-Cc, d	Oropharynx	256	16	+					+	+	+		+
17d	Oropharynx	64	0.5		+
18d	Oropharynx	128	2	+									+		+
19d	Oropharynx	256	0.5	+									+	+
Fluconazole-susceptible isolatesb
ATCC 10231	UNa	0.125	0.008
ATCC 90028	Blood	025	0.03
20	Blood	0.25	<0.008			+											+
21	Oropharynx	0.12	0.008	+		+
22	Blood	0.5	0.016
23	Blood	0.5	0.016
24	Blood	0.25	0.008
25	Blood	0.25	0.008			+												+
26	Blood	0.5	0.008	+		+
27	Blood	0.5	0.008	+		+
28	Blood	0.25	<0.008			+											+
29	Skin	1	0.016	+									+
30	Peritoneal fluid	1	2	+		+
31	Blood	0.12	0.125	+		+												+
32	Blood	0.125	0.008	+									+
33	Blood	0.5	0.008	+		+												+
34	Tissue	0.25	0.008	+									+
35	Blood	2	0.008			+												+
36	Blood	0.25	0.016			+												+
37	Liver	0.5	<0.008			+												+
38	Blood	0.25	<0.008	+		+												+
39	Blood	0.125	<0.008			+
40	Bone	0.25	<0.008	+		+

The "+" sign denotes the presence of the mutation.

^aAbbreviations: RCA, rolling circle amplification; FLU, fluconazole; VOR, voriconazole; UN, unknown.

^b Isolates from the Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney.

^c The "A" and "B" notation of patient numbers refers to isolates cultured sequentially from the same patient at different times.

^dIsolates obtained from the Mycology Unit, Women's and Children's Hospital, Adelaide.