Figure 3.

DCP5 Is Required for mRNA Decapping in Vivo.

(A) Transcript accumulation in dcp5-1. Individual transcript (labeled on the left) levels in control and dcp5-1 at different time points after cordycepin treatment, as shown by RNA gel blot using specific DNA probes. Estimated half-life (min) of specific mRNAs is shown to the right of each panel. Ethidium bromide–stained rRNA bands (bottom panel) are shown as loading controls.

(B) Modified RACE-PCR to detect capped EXPL1 transcripts in wild-type and different mutant plants.