Figure 5.

DCP5 Is Required for Translation Repression of OLEO1/2 mRNAs.

(A) Transcript levels of OLEO1 and OLEO2 in 6-d-old plants were determined by RT-PCR (qRT-PCR results are shown in Table 1). Actin2 levels were used as loading controls.

(B) OLEO1 and OLEO2 levels in 6-d-old plants were measured by protein gel blots using specific antibody. A nonspecific band was used as a loading control.

(C) Effects of DCP5 and DCP5-L on OLEO1 mRNA translation in vitro. MBP-DCP5 and MBP-DCP5-L were added to a wheat germ extract translation system containing the same amount of OLEO1 mRNA. Amounts of proteins are indicated above each lane. Arrowhead indicates the OLEO1 protein.