Figure 10.

Accumulation of Single-Stranded gusA DNA in Tobacco BY-2 Protoplasts following Cotransfection with HTA1.

Tobacco BY-2 protoplasts were cotransfected with single-stranded gusA DNA and either a histone cDNA expression cassette or an empty vector control plasmid. At 0 and 24 h after transfection, samples were treated with DNase I and DNA was extracted. PCR was conducted to determine the amount of gusA DNA within the cell, relative to actin DNA. Error bars indicate se of two or three biological replicates.