Figure 6.

Overexpression of HTA1 in Stably Transformed Tobacco BY-2 Cells Does Not Increase Activity of a Previously Integrated gusA Gene.

Protoplasts isolated from a BY-2 line stably expressing a gusA gene were transfected with either a control empty vector or HTA1 expression constructions. After 24 h, the cells were stained with X-gluc, and the percentage of cells showing GUS activity was determined. Numbers represent GUS activity relative to the control. More than 1000 cells were examined for each treatment. Error bars indicate se of three biological replicates.