Figure 7.

Rate of Accumulation of gusA Double-Stranded DNA in Tobacco BY-2 Protoplasts Cotransfected with Either a HTA1 cDNA or an Empty Vector Control Plasmid.

Protoplasts were electroporated with a gusA transgene, plus either an empty vector or a HTA1 expression construction. After various periods of time, samples were treated with DNase I, following which DNA was extracted. PCR was conducted to determine the amount of gusA DNA within the cell and normalized to the amount of actin DNA. Error bars indicate se of three biological replicates.