Figure 9.

Overexpression of a HTA1 cDNA Can Increase Expression of Various Forms of a Coelectroporated gusA DNA.

Tobacco BY-2 protoplasts were cotransfected with various conformations of a gusA reporter gene and either a histone cDNA expression cassette or an empty vector control plasmid. After 24 h, the percentage of cells expressing GUS activity (X-gluc staining) was determined. The data are presented as the percentage of stained cells relative to that of the control.

(A) Single-stranded gusA transgene.

(B) Double-stranded gusA transgene.

(C) Super-coiled gusA transgene. More than 1000 cells were examined for each treatment. Error bars indicate se.