Figure 2.

Sequence and Complementation Test of ART1.

(A) Amino acid sequence of ART1. Predicted C2H2 domain (red frame with underline), putative nuclear targeting signal (black frame), mutation site (reversed triangle), and defect region in art1 due to frame shift by 1-bp deletion (blue letters) are shown.

(B) Complementation test. Relative root elongation of the wild type, art1, transgenic rice with empty vector (vector control), and three independent transgenic lines with ART1 genomic region (lines 1 to 3) were measured. Data are means ± sd (n = 13 to 15 biological replicates). Different letters indicate significant differences at P < 0.05 by Tukey's test.

(C) Phylogenic tree of ART1-like C2H2 zinc finger proteins in rice (Os-) and Arabidopsis (At-). The sequence alignment used to generate the phylogeny is presented in Supplemental Data Set 1 online. Bootstrap values from 1000 trials are indicated. The 0.1 scale shows substitution distance.

[See online article for color version of this figure.]