Expression of NOV in Seedlings, Leaf Primordia, and Roots.
(A) to (F) Expression of NOVpro:NOV:GUS ([A] and [D] to [F]) and NOVpro:NOV:GFP ([B] and [C]) in seedlings. A whole seedling (A) and leaf primordia ([B] to [F]). Images in (B) to (D) were taken from the lateral view of leaf primordia. Leaf primordia shown in (A) to (E) and in (F) are of the first and second rosette leaves and of the first to fourth rosette leaves, respectively.
(G) to (N) Expression of NOVpro:NOV:GUS ([G] and [H]) and NOVpro:NOV:GFP ([I] to [N]) in roots. Primary root tips ([G] to [I]) and lateral root primordia ([J] to [N]). (H) is a dark-field image of the sample in (G). In (G) and (H), cells of the quiescent centers are marked by asterisks. The root tip in (I) was stained with propidium iodide (magenta) for detecting cell boundary. Samples were taken from seedlings grown for 3 d (B), 3.5 d (C), 4 d (A), 5 d ([D], [E], and [I]), 7 d ([F] to [H] and [L] to [N]), and 8 d ([J] and [K]) after germination. cot, cotyledon; hyp, hypocotyl; lp, leaf primordium; lrp, lateral root primordium; m, shoot apical meristem; st, stipule.
Bars = 10 μm in (B), (C), and (J) to (N), 20 μm in (D), (E), and (G) to (I), and 0.2 mm in (F).