Figure 6.
Biochemical Role of OWL1.
(A) OWL1 accumulation is not affected in different phyA signaling mutants. Seedlings were grown under VLFR conditions for 3 d (FRp; 0.5 μmol m−2 s−1, 5 min/h) and OWL1 detected immunologically. Coomassie blue–stained gel is shown as loading control. Quantified intensity of protein gel blot corrected with the loaded protein amount is given as a mean from three independent blots and sd is added.
(B) The stability of phyA under R light (10 μmol m−2 s−1) is not changed in the owl1 mutant compared with Col. PhyA was detected immunologically between 0 and 120 min of treatment. Coomassie blue–stained gel is shown as loading control. Percentage of accumulation relative to time 0 is given.