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. 2009 Oct;21(10):3226–3244. doi: 10.1105/tpc.109.067876

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Copyright © 2009, American Society of Plant Biologists

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Figure 10. — Transphosphorylation between phot1 Molecules in the Presence or Absence of LOV1.

(A) Autoradiograph showing light-dependent transphosphorylation of a kinase-inactive version of phot1 (phot1^D806N) by GST-phot1 and vice versa in protein extracts from insect cells. Immunoblot analysis of phot1 protein levels is shown below.

(B) Autoradiograph showing light-dependent transphosphorylation of a kinase-inactive version of phot1 (phot1^D806N) by the phot1^LOV2K in protein extracts from insect cells. Immunoblot analysis of phot1 protein levels is shown on the right.

(C) Fluorescence images of a kinase inactive version of phot1-GFP (phot1-GFP^D806N) coexpressed with nonfluorescently labeled phot1^LOV2K in tobacco leaf epidermis show that phot1^LOV2K can induce light-dependent internalization of phot1-GFP^D806N (indicated by white arrows). Bar = 20 μm.

[See online article for color version of this figure.]