Figure 11.

Pharmacological Interference of phot1-GFP Localization in Arabidopsis.

(A) Confocal image of phot1-GFP in 3-d-old etiolated Arabidopsis hypcotyl cells treated with 50 μM BFA. BFA induces internalization and aggregation of phot1-GFP from the plasma membrane into BFA compartments. The white arrows indicate BFA aggregation. Bar = 20 μm.

(B) and (C) Treatment with 30 μM Tyrphostin A23 (Tyr23) inhibits blue light–induced phot1-GFP internalization in the hypocotyls of 3-d-old etiolated Arabidopsis seedlings (B) but does not abolish internalization of the endocytic marker FM4-64 (C) as indicated by the white arrows.

(D) Treatment with 30 μM Tyrphostin A51 (TyrA51), an inactive analog of Tyrphostin A23, has no effect on the blue light–dependent internalization of phot1-GFP from the plasma membrane of hypocotyl cells (as indicated by the white arrows).

[See online article for color version of this figure.]