Figure 8.

Identification of Phosphorylation Sites Regulating phot1 Internalization.

(A) The LOV2-kinase region of phot1 (phot1-GFP^LOV2K) undergoes blue light–dependent relocalization (indicated by the white arrows).

(B) Mutation of Ser-849 to Ala in phot1^2xLOV1 does not impair internalization in darkness (indicated by white arrows).

(C) Mutation of Ser-851 to Ala in phot1^2xLOV1 severely impacts the formation of cytosolic structures in darkness.

(D) Mutation of both Ser-849 and Ser-851 to Ala in phot1^2xLOV1 impairs internalization in darkness.

(E) Mutation of Ser-851 to Asp in phot1-GFP induces the formation of cytosolic structures in darkness (indicated by white arrows).

Bars = 20 μm.

[See online article for color version of this figure.]