Table 1.

Kinetic Parameters of Recombinant LOG and At LOG Proteins.

Enzyme	Km	Vmax	kcat	kcat/Km	Sp. Ratioa	Optimum pH
	μM	Units mg−1 Protein	min−1	min−1 M−1	%
At LOG1	16 ± 2	2.1 ± 0.1	50	3.2 × 106	26	6.5
At LOG2	58 ± 15	14 ± 2	328	5.6 × 106	45	6.5
At LOG3	14 ± 2	1.5 ± 0.0	34	2.5 × 106	20	6.5
At LOG4	8.6 ± 0.6	4.4 ± 0.2	103	1.2 × 107	97	6.5
At LOG5	11 ± 1	0.73 ± 0.03	19	1.6 × 106	13	5.4
At LOG7	6.7 ± 0.8	3.8 ± 0.2	91	1.4 × 107	110	6.5
At LOG8	17 ± 1	0.053 ± 0.009	1.3	7.7 × 104	0.6	7.0
LOG	12 ± 1b	5.6 ± 1.1b	145	1.2 × 107	100	6.5b

The kinetic parameters for iPRMP were measured at the optimum pH for each enzyme. Means ± sd (n = 3).

^aSp. Ratio is the specificity ratio, which is the relative k_cat/K_m with the value of LOG taken as 100%.

^bKurakawa et al. (2007).