Figure 2.

U. maydis PMTs Complement S. pombe ogm1Δ and ogm4Δ Mutants.

(A) Functional complementation of S. pombe ogm1Δ. Serial dilutions of wild-type S. pombe leu1-32 strain transformed with pREP41X and the ogm1Δ strain transformed with either pREP41X (control), pREP41X-Umpmt1, pREP41X-Umpmt2, or pREP41X-Umpmt4 are shown after 3 d at 28 or 36°C. ogm1Δ cells were viable at 36°C if transformed with U. maydis pmt1. Um pmt1, pmt2, and pmt4 were able to partially complement the ogm1Δ mutant at 28°C.

(B) S. pombe ogm4Δ complementation. The ogm4Δ mutant cells form aggregates. The ogm4Δ mutants transformed with pREP41X LEU2 (empty vector) or with the vector containing either pmt1, pmt2, or pmt4 were grown to exponential phase at 28°C and photographed. pmt4 complemented the phenotype of the ogm4Δ mutant resulting in single cell growth.

(C) Quantification of pmt4 functional complementation of ogm4Δ. The quantification shows the percentage of aggregates relative to individual cells. Three independent samples were analyzed for each strain (number of cells for each sample ≥100). Error bars represent se of the mean.