Table 2.

Quantification of Appressoria and Plant Penetration

Inoculum	No. of Appressorium-Mediated Penetrations/No. of Appressoria	Percentage	Distance from Hyphal Tip to the Closest Visible Septum (μm)	n
FB1 × FB2	96/97	98.97	6.15 ± 1.13	49
FB1 Δgas1 × FB2 Δgas1	46/47	97.87	10.97 ± 3.06	29
FB1 Δpmt4 × FB2 Δpmt4	0/73	0.00	17.62 ± 4.56	47

Maize seedlings were infected with the strains indicated above. Two days after infection, leaf samples were stained with Chlorazole Black E and analyzed by light microscopy. Analysis was based on assays of three independent pools of plants with the total number of appressoria scored shown (n).