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. 2009 Aug 25;101(Pt 11):645–659. doi: 10.1042/BC20090042

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A single notochord precursor blastomere was injected with Wnt5α MO or mRNA, or with Dsh MO. Examples of normal and abnormal intercalation of the labelled cells (asterisks) in the two situations where labelled cells are present in isolation or two labelled cells are adjacent. Abnormal positioning of labelled cells is categorized into two and four types respectively. The detailed frequency of each abnormality is shown is Table 2.