Table 2. Intercalation of notochord cells in embryos in which Wnt-5α/Dsh antisense MOs or mRNA were injected into notochord blastomeres.
Relative positions of labelled cells within the notochord are indicated in red. Isolated single cell: labelled cells are present in isolation. Neighbouring two cells: two labelled cells are adjacent. n=number of individual labelled notochord cells counted. Representative specimen of each normal or abnormal case is shown in Figure 7. Note that the results obtained from injection of Wnt5α MO or mRNA, or of Dsh MO, are quite similar. Concentrations of the injected solutions are indicated in parentheses.
| Isolated single cell | Neighbouring two cells | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Intercalation (%) | Intercalation (%) | |||||||||
| Normal | Failed | Normal | Failed | |||||||
| n |  |
 |
 |
n |  |
 |
 |
 |
 |
|
| Wnt-5α MO (10–20 μg/μl) | 48 | 77 | 6 | 17 | 28 | 11 | 11 | 11 | 7 | 61 |
| Total 23 | Total 89 | |||||||||
| Wnt-5α mRNA (0.8–3.6 μg/μl) | 104 | 76 | 12 | 13 | 42 | 21 | 21 | 26 | 14 | 17 |
| Total 24 | Total 79 | |||||||||
| Dsh MO (5–10 μg/μl) | 52 | 79 | 4 | 17 | 14 | 14 | 21 | 0 | 21 | 43 |
| Total 21 | Total 86 | |||||||||