Figure 2. The oxidized peptide does not inhibit phosphorylation of the reduced SP49 peptide by CDPKβ.

Increasing amounts of the SP49Mox peptide [KGR(MetSO)RRISSVEJJK] were added to complete phosphorylation reaction mixtures containing the SP49M peptide (KGRMRRISSVEJJK). Total incorporation of [³²P] was monitored with the SP49M peptide alone (white bar) or with an equivalent amount or 2-fold excess of the SP49Mox peptide (grey bars), or with the SP49Mox peptide alone (black bars). Values are means±S.E.M. (n=3) from a representative experiment.