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. 2009 Jul 29;422(Pt 1):139–149. doi: 10.1042/BJ20082308

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) RP-HPLC of standard 1, 8-Br-NHD and 8-Br-N¹-cIDPR (8-Br-cIDPR). (b) RP-HPLC of standard 2, nicotinamide (NicAmid), 8-Br-NHD and 8-Br-IDPR. 8-Br-IDPR was synthesized by incubation of 8-Br-NHD with NADase of Neurospora crassa. 1 mM 8-Br-NHD was incubated with 1 μg/μl ADP-ribosyl cyclase of A. californica in 25 mM Hepes, pH 7.4, for 15 h at room temperature. Aliquots were taken at (c) 0 h and (d) 15 h and analysed by RP-HPLC. (e) RP-HPLC of purified 8-Br-N¹-cIDPR.