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. 2009 Jul 29;422(Pt 1):139–149. doi: 10.1042/BJ20082308

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Jurkat T-cells were incubated with 3 mM 8-Br-N¹-cIDPR for 5 min at room temperature. Cellular nucleotides were extracted with TCA. Crude cell extracts were purified by solid-phase extraction and analysed by RP-HPLC. (a) Representative RP-HPLC analysis of absorbed 8-Br-N¹-cIDPR in Jurkat T-cells is shown (black line). (b) Magnification of the trace shown in (a). The peak of 8-Br-N¹-cIDPR is indicated by an arrow. As a control, cell extracts of cells not incubated with 8-Br-N¹-cIDPR were analysed by HPLC (grey line). Jurkat T-cells incubated with 8-Br-N¹-cIDPR took up 0.16 fmol per cell with a recovery of about 88%. Results are means (n=2).