Figure 4. Extracellular addition of 8-Br-N¹-cIDPR evoked Ca²⁺ signalling in intact Jurkat T-cells.

Jurkat T-cells were loaded with Fura-2AM and subjected to Ca²⁺ imaging. The time points of addition of (a) buffer, (b) 1 mM N1-cIDPR and (c) 1 mM 8-Br-N¹-cIDPR are indicated by arrows. Characteristic tracings from a representative experiment are shown. (d) Concentration–response curve of 8-Br-N¹-cIDPR. Results represent means±S.E.M. (n=21–109) of single tracings from time points 100–400 s (of the 8-Br-N¹-cIDPR mediated Ca²⁺ peak). *P< 0.01 and **P<0.001.