Figure 5. Extracellular addition of 8-Br-N¹-cIDPR evoked Ca²⁺ signalling in primary rat T-cells.

Rat T-cells were loaded with Fura-2AM and subjected to Ca²⁺ imaging. The time points of addition of (a) buffer, (b) anti-CD3 followed by a cross-linking IgM (40 μg/ml and 10 μg/ml) and (c) 1 mM 8-Br-N¹-cIDPR are indicated by arrows. Characteristic tracings from a representative experiment are shown. (d) Combined results representing means±S.E.M (n=186–241) of single tracings from time points 100–400 s (the Ca²⁺ peak). **, P<0.001 (t test).