Figure 6. 8-Br-N¹-cIDPR activated both Ca²⁺ release and Ca²⁺ entry in intact Jurkat T-cells.

Jurkat T-cells were loaded with Fura-2AM and subjected to Ca²⁺ imaging. (a) Cells were kept in a nominally Ca²⁺ free buffer in the first part of experiment and 8-Br-N¹-cIDPR was added as indicated. Then, CaCl₂ was re-added. (b) Buffer was added instead of 8-Br-N¹-cIDPR as a control. The time points of addition of buffer, 1 mM 8-Br-N¹-cIDPR or 1 mM Ca²⁺ are indicated by arrows. Characteristic tracings from representative experiments are shown. (c) Combined data representing means±S.E.M. (n=362–404) of single tracings from time points 100–200 s (8-Br-N¹-cIDPR mediated Ca²⁺ release-peak) or 300–450 s (8-Br-N¹-cIDPR mediated Ca²⁺ entry-peak). **, P<0.001 (t test).