Figure 7. Effect of RuRed on 8-Br-N¹-cIDPR mediated Ca²⁺ signalling in intact Jurkat T-cells.

Jurkat T-cells were loaded with Fura-2AM and subjected to combined Ca²⁺ imaging and microinjections. Time points of microinjection are indicated by arrows. Cells were microinjected with (a) intracellular buffer (n=26), (b) 1 mM 8-Br-N¹-cIDPR (n=21) or (c) a mixture of 10 μM RuRed and 1 mM 8-Br-N¹-cIDPR (n=20). (d) Combined results representing means±S.E.M. of single tracings from time points 50–150 s (the Ca²⁺ peak). **, P< 0.001 (t test). ns, not significant.