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. 2009 Sep 23;158(5):1183–1195. doi: 10.1111/j.1476-5381.2009.00389.x

Figure 5.

Figure 5

γ-Secretase inhibitor (GSI) altered mitochondrial transmembrane potential (ΔΨm) and facilitated cytochrome c release in TALL-1 cells. Cells were treated with either dimethyl sulphoxide (DMSO) or 1 µM GSI for 2 days and harvested on days 1, 3 and 7 after the last addition of drug. (A) Cells treated with GSI or DMSO were stained with JC1 dye and analysed by flow cytometry. (B) Cytosol without mitochondria (S-100 fraction) was prepared from cells treated with either DMSO or 1 µM of GSI, and immunoblot analysis was performed using antibodies against cytochrome c and β-actin. Lower panel, ratio of cytochrome c to β-actin was determined by densitometric analysis.