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. 2009 Sep 28;158(5):1236–1247. doi: 10.1111/j.1476-5381.2009.00401.x

Figure 5.

Figure 5

Effects of gly-rhAFP (100 µg·mL−1) and UVA (2.5 J·cm−2) on intracellular NO levels and iNOS gene expression in HaCaT cells (mean ± SD). (A) Typical fluorescence micrographs of HaCaT cells incubated for 1 h with fluorescent probe DAF-2DA: 1 – control cells; 2 – cells exposed to gly-rhAFP; 3 – cells exposed to UVA, 4 – cells exposed to the combination of UVA and gly-rhAFP. (B) Fluorescence intensity (F530·mg−1 protein) in the lysates of DAF-2DA-loaded HaCaT cells. (C) iNOS gene expression expressed as fold induction related to control. *P < 0.05; **P < 0.01 versus control. AFP, α-fetoprotein; DAF-DA, 4,5-diaminofluorescein diacetate; gly-rhAFP, glycosylated recombinant human alpha fetoprotein; iNOS, inducible nitric oxide synthase.