Figure 7.

Gly-rhAFP activates AP-1 in normal human keratinocytes. (A) Western blot analysis of c-Fos (AP-1 subunit) and p65 (RelA, NFκB subunit) in nuclear lysates (10 µg per lane) from untreated cultures (-), incubated for 20 min with 50 or 10 µg·mL⁻¹ rhAFP alone, or in the presence of 50 ng·mL⁻¹ TNF-α or 50 U·mL⁻¹ IFN-γ. Lamin B was used to assess equal loading. These results are representative of three independent experiments. (B) AP-1- and NFκB-specific DNA binding activity in keratinocytes following 2 h treatments. **P < 0.01 versus untreated keratinocyte cultures (-).*P < 0.05 versus untreated keratinocyte cultures. AFP, α-fetoprotein; AP-1, activator protein 1; gly-rhAFP, glycosylated recombinant human alpha fetoprotein; IFN-γ, interferon gamma; NFκB, nuclear factor kappa B; TNF-α, tumour necrosis factor alpha.