Table 2.
Effects of gly-rhAFP (100 µg·mL−1) and UVA (2.5 J·cm−2) on the release of cytokines (pg·mg−1 protein) from HaCaT cells within 24 h
| Cytokines | Control | AFP | UVA | UVA+AFP |
|---|---|---|---|---|
| IL-1β | 0.5 ± 0.02 | 1.4 ± 0.07**a | 0.8 ± 0.21 | 1.5 ± 0.22**a |
| IL-1ra | 4 ± 1 | 18 ± 1***a | 12 ± 7*a | 19 ± 3***a |
| IL-6 | 1.1 ± 0.0 | 4.0 ± 0.2**a | 1.6 ± 0.5 | 5.0 ± 0.5**a |
| IL-8 | 6 ± 0 | 16 ± 1***a | 8 ± 1 | 16 ± 2**a |
| IL-10 | 279 ± 19 | 933 ± 86**a | 441 ± 105 | 1063 ± 207**a |
| IP-10 | 4 ± 1 | 13 ± 2**a | 4 ± 2 | 11 ± 3*a |
| MCP-1 | 99 ± 19 | 1195 ± 83**a | 132 ± 54 | 912 ± 84**a,*b |
| MIP-1α | 8 ± 1 | 10 ± 0*a | 10 ± 1 | 12 ± 1 |
| MIP-1β | 1.4 ± 0.1 | 3.9 ± 0.4**a | 2.4 ± 0.8 | 5.2 ± 1.5**a |
| PDGFbb | 3 ± 0 | 17 ± 2***a | 7 ± 4 | 14 ± 2*a |
| TNF-α | 7 ± 2 | 17 ± 2**a | 9 ± 4 | 13 ± 3**a |
| VEGF | 1938 ± 182 | 7432 ± 335***a | 2940 ± 835*a | 7698 ± 759***a |
Values shown in the Table are means ± SD from three independent experiments.
AFP, α-fetoprotein; gly-rhAFP, glycosylated recombinant human alpha fetoprotein; IL, interleukin; IL-1ra, interleukin 1 receptor antagonist; IP-10, interferon gamma-produced protein of 10 kDa; MCP-1, monocyte chemotactic protein 1; MIP, macrophage inflammatory protein; PDGF, platelet-derived growth factor; TNF-α, tumour necrosis factor alpha; VEGF, vascular endothelial growth factor.
*a
P < 0.05;
**a
P < 0.01;
***a
P < 0.001 versus control cells;
*b
P < 0.05 versus cells exposed to gly-rhAFP.