Table 4.

Effects of gly-rhAFP on the activity of catalase, SOD, GST, and the level of GSH and protein SH-groups in HaCaT cells (mean ± SD)

Gly-rhAFP, µg·mL−1	Catalase	GST	SOD	GSH	Protein SH-groups
	µmol·min−1·mg−1protein		U·mg−1protein	nmol·mg−1protein
0a	1.20 ± 0.28	0.94 ± 0.27	3.50 ± 0.77	203 ± 26	19.6 ± 10.4
25a	1.28 ± 0.34	0.92 ± 0.17	3.67 ± 0.48	201 ± 18	21.3 ± 16.1
50a	1.48 ± 0.38	0.98 ± 0.27	3.86 ± 0.82	209 ± 15	17.3 ± 20.0
100a	1.24 ± 0.35	0.94 ± 0.29	3.42 ± 0.71	194 ± 23	21.9 ± 21.1
0b	1.07 ± 0.21	1.02 ± 0.19	3.44 ± 0.28	241 ± 29	24.8 ± 16.0
25b	0.93 ± 0.21	0.90 ± 0.13	3.22 ± 0.22	228 ± 37	21.6 ± 9.1
50b	0.97 ± 0.22	0.95 ± 0.18	3.38 ± 0.33	236 ± 29	25.5 ± 23.2
100b	1.14 ± 0.13	1.11 ± 0.40	3.37 ± 0.58	232 ± 41	25.7 ± 19.2
0c	2.28 ± 0.80	1.03 ± 0.08	3.38 ± 0.06	184 ± 34	17.4 ± 5.0
25c	2.01 ± 0.57	1.07 ± 0.25	4.43 ± 1.94	177 ± 9	26.0 ± 13.0
50c	1.86 ± 0.43	0.89 ± 0.01	4.06 ± 0.60	181 ± 20	19.7 ± 8.0
100c	2.24 ± 0.78	1.09 ± 0.21	4.19 ± 0.72	199 ± 27	27.2 ± 18.0

Incubation time with gly-rhAFP:

^a1 h;

^b4 h;

^c24 h.

Values shown in the Table are means ± SD from four–five independent experiments.

gly-rhAFP, glycosylated recombinant human alpha fetoprotein; GST, glutathione-S-transferase; SOD, superoxide dismutase.