Table 2.

Effect of NaHS on the fura-2 ratio signal

	ΔFura-2 ratio induced by NaHS
	Initial decrease	Late increase	n
Control	−0.102 ± 0.0069a	0.161 ± 0.014a	224
Ca2+-free	−0.062 ± 0.0077b	0.145 ± 0.019a,b	86
+2-APB	−0.099 ± 0.0095a	0.197 ± 0.022a	108
+Ruthenium red	−0.003 ± 0.0009c	0.056 ± 0.017b	82
+Cyclopiazonic acid	−0.035 ± 0.0066b,c	0.347 ± 0.045c	84

Effect of NaHS (10⁻⁴ mol·L⁻¹) in the absence of any inhibitors (control), in the nominal absence of extracellular Ca²⁺, or in the presence of 2-aminoethoxydiphenylborate (2-APB; 10⁻⁵ mol·L⁻¹), ruthenium red (5 × 10⁻⁵ mol·L⁻¹), or cyclopiazonic acid (10⁻⁵ mol·L⁻¹). The response to NaHS is given as initial decrease (minimal ratio within 4 min after administration of NaHS) and secondary increase (maximum reached after administration of NaHS). Values are given as difference from the baseline in the fura-2 ratio just prior to administration of NaHS and are means ± SEM. Statistically homogeneous groups are indicated by the same letter

^a–canalysis of variances followed by Tukey's test).