Figure 2.

Channel modulation by the neuroactive steroids. The wild-type α1β2γ2L receptors were activated by 5 µM GABA (∼EC₂₅) in the absence and presence of 1 µM 3α5α18nor17βCN (A), 1 µM 3α5αCDNC12 (B), 3 µM 3α5α17βEt (C) or 3 µM 3α5α17H (E). The α1N407A/Y410F double mutant receptor was activated by 10 µM GABA (∼EC₂₅) in the absence and presence of 3 µM 3α5α17βEt (D). All steroid analogues potentiated the wild-type receptor. Exposure to 3α5α17βEt did not modulate the current response from receptors containing the α1N407A/Y410F double mutation. Note the lack of wash-out following exposure to 3α5αCDNC12, 3α5α17βEt or 3α5α17H.