Summary of single-channel kinetic analysis of the closed time distributions from the α1β2γ2L receptor under control conditions, and in the presence of steroids

Agonist, modulator	CT1 (ms)	Fraction CT1	CT2 (ms)	Fraction CT2	CT3 (ms)	Fraction CT3	n
50 µM GABA	0.15 ± 0.01	0.59 ± 0.09	1.4 ± 0.3	0.13 ± 0.04	13.6 ± 4.0	0.28 ± 0.06	5
+1 µM 3α5αP	0.22 ± 0.04*	0.64 ± 0.12ns	1.4 ± 0.2ns	0.30 ± 0.10*	14.3 ± 1.2ns	0.05 ± 0.01***	4
+1 µM 3α5α18nor17βCN	0.16 ± 0.05ns	0.65 ± 0.09ns	1.1 ± 0.5ns	0.22 ± 0.06**	11.3 ± 2.8ns	0.13 ± 0.04***	4
+3 µM 3α5αCDNC12	0.16 ± 0.03ns	0.60 ± 0.03ns	1.1 ± 0.2ns	0.30 ± 0.02***	11.1 ± 2.4ns	0.11 ± 0.02***	4
+10 µM 3α5α17βEt	0.23 ± 0.04ns	0.67 ± 0.06ns	1.3 ± 0.2ns	0.21 ± 0.03*	14.8 ± 5.0ns	0.12 ± 0.05***	4
+10 µM 3α5α17H	0.26 ± 0.06**	0.63 ± 0.04ns	1.3 ± 0.1ns	0.32 ± 0.03***	11.0 ± 2.3ns	0.05 ± 0.01***	6

The intracluster closed time histograms were fitted to a sum of three exponentials. The table gives the mean durations ± SD (CT1-3) and average relative contributions ± SD (fraction CT1-3) for the three closed time components, and the number of patches under each condition (n). Statistical analysis was carried out using analysis of variance with Dunnett's correction (Systat 7.0, Systat Software, Inc., Point Richmond, CA, USA). The significance levels apply to comparison to control condition (50 µM GABA). The data for 3α5αP are from Akk et al. (2008).

^*P < 0.05;

^**P < 0.01;

^***P < 0.001; ns, not significant.