The effect of 24 h FD on (A) blood THC and THC-COOH concentrations and (B) plasma glycerol in rats (300–400 g) that were repeatedly pretreated with THC (10 mg·kg−1·day−1) for 10 days before a 2 day washout period (n= 10 per group). The effect of 24 h FD on (C) blood THC and THC-COOH concentrations and (D) plasma glycerol in rats that were repeatedly pretreated with THC (10 mg·kg−1·day−1) for 10 days and given a 7 day washout period (n= 7–8 per group). *P < 0.05, **P < 0.01 and ***P < 0.001, significant difference between FD and NFD group. FD, food deprivation; NFD, non-food deprived; THC, Δ9-tetrahydrocannabinol; THC-COOH, (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol.