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Two-channel scans performed continuously on the same track consisting of antibody conjugated with fluorescein after a reverse-phase assay. The interferometry wavelength is 488 nm, and the fluorescence wavelength is 510 nm. (a) shows the time-course scanning results on both channels as a function of position and time. The fluorescence becomes weaker with time (increasing downward) due to bleaching. (b) shows the signal intensity variations. From Ref. 123.
