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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Dev Dyn. 2009 Aug;238(8):2044–2057. doi: 10.1002/dvdy.22028

Figure 6. Divergent, gene-specific somite defects in Dvl morphants.

Figure 6

Lateral confocal projections displaying somite morphology of Dvl morphants with a muscle specific mouse anti-12/101 antibody (green) and the somite boundaries with a mouse anti-fibronectin antibody (red). (A): Normal morphology of the medial flank of a stage 29/30 embryo (B): Normal fibronectin deposition in somite boundaries stage 29/30 embryo. (C): Dvl1 morphant stage 29/30 embryo displaying segmentation defect (white arrow) (D): Dvl1 morphant stage 29/30 embryo displays defects in the deposition of fibronectin (red) while “chevron” shape is conserved. (E): Dvl3 morphant stage 29/30 embryo displaying un-“chevroned” somites and multiple cells are disrespecting the established somite boundaries. (F): Dvl3 morphant stage 29/30 embryo displaying normal deposition of fibronectin between un-“chevroned” boundaries. Somite defects were observed in 50-90% of morphants, depending upon the experiment.