Table 1. Complementary base-pairing of Dvl genes to MO used in this study.

Number indicates the number of mismatched base-pairs for each MO and each gene. Green values indicate that the MO should effectively target the gene. Red values indicate 4- or 5- Bp mismatches between a given MO and a given Dvl gene mRNA sequence. These can serve as controls of MO specificity. (Note: For Dvl2 and Dvl3, our the sequences targeted by the MOs are perfectly conserved for both alloalleles, as identified from EST data; for Dvl1, only a single EST exists.)

Gene →	Dvl1	Dvl2	Dvl3
Morpholinos:
Dvl1	0	5	4
Dvl1 MM	5	7	9
Dvl2	9	0	9
Dvl3	4	5	0
Dvl3B	12	11	0
Dvl3 MM	9	7	5