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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: J Sep Sci. 2009 May;32(10):1507–1522. doi: 10.1002/jssc.200800685

Figure 10.

Figure 10

Examples of (a) a typical peak decay curve and (b) a logarithmic transform of this curve as determined for elution of the sugar methylumbilliferyl α-D-mannopyranoside (MUM) from an immobilized concanavalin A column after a step change was made to the continuous application of 0.1 M mannose as a competing agent. These chromatograms were collected on a 6.3 mm × 2.1 mm i.d. column at 10 mL/min. The slope of the curve in (b) was analyzed according to Eq. (13) to obtain the dissociation rate constant for MUM from this column. (Adapted with permission from Ref. [36])