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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Neurobiol Aging. 2007 Dec 21;30(9):1350–1360. doi: 10.1016/j.neurobiolaging.2007.11.014

Figure 2.

Figure 2

The presence of the APOE4 gene promotes induction of NOS2 mRNA and increased production of NO in immune stimulated microglia: Panel A- Average supernatant levels of nitrite (± SEM) were measured for APOE3/3 or APOE4/4 microglial cultures stimulated for 24 hrs with recombinant murine IFNγ (100 U/ml) plus increasing doses of PIC or LPS. * P <0.04 for IFNγ–treated APOE4/4 microglia compared to IFNγ–treated APOE3/3 microglia; ** p <0.0001 for IFNγ + PIC- treated APOE4/4 microglia compared to APOE3/3 microglia; ***p <0.0001 for IFNγ + LPS - treated APOE4/4 microglia compared to APOE3/3 microglia; all using 2-way ANOVA. A significant effect of dose was observed for PIC treatment (p < 0.001; 2 way ANOVA) but was not observed for LPS treatment. Panel B- Average supernatant levels of nitrite (± SEM) were measured for APOE3/3 or APOE4/4 microglial cultures stimulated for 24 hrs with recombinant murine IFNγ (100 U/ml) + LPS (100 ng/ml) in media containing physiological levels of arginine (10 µm). * p< 0.001 for APOE4/4 compared to APOE3/3 microglia. Panel C- Relative changes in mRNA for NOS2 was determined for LPS (10 ng/ml) + IFNγ (10 U/ml)-treated APOE3/3 microglia compared to APOE4/4 microglia. A significant increase (p < 0.03; unpaired student’s t test) was observed after 6 hrs of treatment in APOE4/4 compared to APOE3/3 microglia. Panel D- A significant decrease (***p < 0.001; 2-way ANOVA) in supernatant nitrite levels was found in both APOE3/3 and APOE4/4 microglia treated with LPS (100 ng/ml) + IFNγ (100 U/ml) in the presence of varying doses of 1400W, an iNOS inhibitor. No effect of 1400W alone was observed. #= p < 0.01 for LPS + IFNγ-treated APOE4/4 microglia compared to APOE3/3 microglia.