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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Neurobiol Aging. 2007 Dec 21;30(9):1350–1360. doi: 10.1016/j.neurobiolaging.2007.11.014

Figure 4.

Figure 4

Innate immune responses are also increased in cultured peritoneal macrophages derived from adult male APOE4/4 mice. Panel A- Average supernatant levels of nitrite (± SEM) were measured for APOE3/3 or APOE4/4 peritoneal macrophage cultures stimulated for 24 hrs with recombinant murine IFNγ (100 U/ml) plus increasing doses of PIC or LPS. Using 2-way ANOVA, a significant effect of APOE4 genotype (* p < 0.001) was observed for all treatment conditions while a significant effect of dose (p < 0.01) was observed for PIC treatment. Panel B- Average supernatant levels of TNFα (± SEM) were measured after 24 hrs in immune stimulated APOE3/3 and APOE4/4 peritoneal macrophage cultures using ELISA. LPS = 100 ng/ml; IFNγ= 100 U/ml. In each case, a significantly higher level of TNFα was found in APOE4/4 cultures compared to APOE3/3 ( * = p < 0.001; 2 way ANOVA).