Figure 3.

Acj6 sites are required for expression of Or genes. A, B, GFP expression driven by wild-type Or85e- and Or46a-GAL4 constructs (top panels) and constructs with mutations in predicted Acj6 sites (bottom panels). The proximal Acj6 site in Or85e, which is likely to lie in the 5′-untranslated region of the gene, is not shown. Data were from four independent transgenic lines for the mutant Or85e construct, and three lines for the mutant Or46a construct. C, The effects of mutations in Acj6 sites in Or42a. Data were pooled from three independent transgenic lines for each mutated construct. n = 11 for wild type; 30 < n < 37 for mutated constructs. *p < 0.05; **p < 0.01; ****p < 0.0001 (one-way ANOVA and Holm–Sidak test).