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Reduction in Ad5 peak area with increasing flow rate under non-adsorbing conditions. Four DEAE CIM disks; us = 0.2 cm/min (········), 0.4 cm/min (- · -), 1 cm/min (- - -), and 2.7 cm/min (—). Pulse and background: 1.5 M NaCl, 20 mM HEPES, 1.7% glycerol, pH 7.8. Concentration of Ad5 in the pulse was 3.5 × 1011 p/mL (0.1 g/L).
