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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: FEMS Microbiol Lett. 2009 Jun 26;298(1):111–117. doi: 10.1111/j.1574-6968.2009.01707.x

Fig. 2. Map of FLP recombinase expressing plasmid.

Fig. 2

The physical map of pBBR1-FLP2 (GenBank accession number FJ797950) shows the locations and orientations of the genetic determinants involved in replication (rep), mobilization (mob), ampicillin/ carbenicillin resistance (bla, β-lactamase-coding gene), FLP-FRT recombination (FLP, Flippase-coding gene) and the counter-selectable marker (sacB, Bacillus subtilis levansucrase-encoding gene). Some unique restriction enzyme sites are shown. cI857, gene encoding a temperature sensitive λ repressor.