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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Dev Comp Immunol. 2007 Dec 26;32(7):795–807. doi: 10.1016/j.dci.2007.11.007

Figure 5. Dual location of Tt-CATL immune reactivity and fluorescent bacteria as observed by confocal microscopy.

Figure 5

Animals were injected with FITC-labelled bacteria (M. luteus or E. coli) and sacrificed after various times. Tt-CATL immune detection was realized using a Texas-Red labelled secondary antibody. An accumulation of Tt-CATL immunopositive granular amœbocytes is observed at the injection site. A. Five minutes after bacterial injection, a few granular amœbocytes only are observed in cœlomic cavities near the injection site. B, C. After 12 hours, strong cathepsin-L immunoreactivity is observed at the injection site. Bars: 100 μm.